A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site.
نویسندگان
چکیده
The small basic protein encoded by the open reading frame adjacent to the terC site in the Bacillus subtilis chromosome and previously implicated in termination of the replication process was purified. Band retardation assays established that this protein (now called the replication terminator protein, encoded by the rtp gene) binds specifically to a 209-base-pair fragment of DNA within which terC is located.
منابع مشابه
DNA and protein sequence conservation at the replication terminus in Bacillus subtilis 168 and W23.
Cloned DNA from the replication terminus region of Bacillus subtilis 168 was used to identify and construct a restriction map of the homologous region in B. subtilis W23. With this information, DNA from the terminus region of W23 was cloned and the sequence was determined for a 1,499-base-pair segment spanning the expected terC site. The position of the site was then located more precisely. Use...
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عنوان ژورنال:
- Journal of bacteriology
دوره 171 6 شماره
صفحات -
تاریخ انتشار 1989